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KMID : 1134820220510040311
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Journal of the Korean Society of Food Science and Nutrition
2022 Volume.51 No. 4 p.311 ~ p.321
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Quercetin Inhibits Adipocyte Differentiation via Matrix Metalloproteinases in 3T3-L1 Preadipocytes
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Song Seung-Min
Jung Soo-Min
Choi Su-Jin
Kim Woo-Kyoung
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Abstract
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The purpose of this study was to investigate the inhibitory effects of quercetin on differentiation and angiogenesis via matrix metalloproteinases (MMPs) in 3T3-L1 preadipocytes. The adipocytes were treated with quercetin (0, 5, 10, and 20 μM) for 24 h and the activation of the MMPs was induced by phorbol 12-myristate 13-acetate (PMA) (100 ng/mL) (PMA+) for 2 h. The mRNA expression and activity of MMP-2 and MMP-9 were measured using a real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay kit, respectively. Oil-Red-O (ORO) staining and glycerol-3-phosphate dehydrogenase (GPDH) activity were measured. Also, the mRNA expressions of CCAAT/enhancer-binding protein (C/EBP) β, C/EBPα, peroxisome proliferator-activated receptor (PPAR) γ, fatty acid-binding protein (FABP) 4, vascular endothelial growth factor (VEGF)-A, and vascular endothelial growth factor receptor (VEGFR)-2 were measured using a real-time PCR. The mRNA expression and activity of MMP-2 and MMP-9 were significantly increased in the quercetin 0 μM (PMA+) group compared to the quercetin 0 μM (PMA?) group, and they were significantly decreased by quercetin treatment (P<0.05). ORO staining and GPDH activity were significantly increased in the quercetin 0 μM (PMA+) group compared to the quercetin 0 μM (PMA?) group, and they were significantly decreased by quercetin treatment (P<0.05). The mRNA expressions of C/EBPβ, C/EBPα, PPARγ, FABP4, VEGF-A, and VEGFR-2 were significantly increased in the quercetin 0 μM (PMA+) group compared to the quercetin 0 μM (PMA?) group, and they were significantly decreased by the quercetin treatment (P<0.05). In conclusion, quercetin can inhibit differentiation and angiogenesis in 3T3-L1 preadipocytes through the suppression of MMPs.
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KEYWORD
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quercetin, adipogenesis, angiogenesis, vascular endothelial growth factor, matrix metalloproteinases
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